84-4424-36　KIBRA (Kidney And Brain Protein WWC1, WW Domain Containing Protein 1, WW,C2 And Coiled-Coil Domain Containing 1, HBeAg-binding protein 3) 200ul　363833

特徴

• The deduced 1113aa protein has a calculated molecular mass of about 125kD.
• It contains 2 N-terminal WW domains, and a C2-like domain and glutamine-rich domain in its C-terminal half.
• Northern blot analysis detected a major 4.3kb transcript predominantly expressed in kidney and brain.
• Following expression in green monkey kidney cells, Western blot analysis detected human KIBRA and endogenous Kibra at an apparent molecular mass of 125kD.
• Fluorescence microscopy localized KIBRA predominantly to the cytoplasm, with perinuclear enrichment.
• Highest expression in liver and kidney, intermediate expression in brain, lung, pancreas, and ovary, weak expression in heart and testis, and no expression in skeletal muscle and spleen.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.