SAM68 is a tyrosine-phosphorylated, SRC-associated protein in mitotic cells.
SAM68 protein contains 3 N-terminal proline-rich sequences, followed by a KH domain embedded within a STAR domain, 3 more proline-rich sequences, a C-terminal tyrosine-rich region, which contains a domain similar to the SRGY domain of RNA-binding proteins, and a putative nuclear localization signal.
SAM68 has a calculated molecular mass of 48.2kD, but it migrated as a 68kD protein on SDS-PAGE gels.
Northern blot analysis detected a 3.1kb SAM68 transcript expressed at moderate levels in heart, brain, placenta, skeletal muscle, kidney, and pancreas, and at lower levels in lung and liver.
The p62/SAM68 gene was phosphorylated when expressed in the presence of v-src, and only the phosphorylated form bound to GAP.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KHDRBS1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu152-Glu381 of human KHDRBS1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
