The KEL gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen.
The Kell glycoprotein links via a single disulfide bond to the XK membrane protein that carries the Kx antigen.
The encoded protein contains sequence and structural similarity to members of the neprilysin (M13) family of zinc endopeptidases.
There are several alleles of the gene which creates Kell protein.
Two such alleles, K1 (Kell) and K2 (Cellano), are the most common.
The kell protein is tightly bound to a second protein, XK, by a disulfide bond.
Absence of the XK protein (such as through genetic deletion), leads to marked reduction of the Kell antigens on the red blood cell surface.
Absence of the Kell protein (K0), however, does not affect the XK protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry:: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse KEL.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ile49-Leu358 of mouse KEL, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
