KEAP1 encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain.
Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus.
This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase.
Two alternatively spliced transcript variants encoding the same isoform have been found for this gene.
Kelch motifs of Keap1 interact with the Neh2 domain of Nrf2 and that this interaction is required for the repression of Nrf2 by Keap1.
Fluorescence microscopy demonstrated that Keap1 is expressed in the cytoplasm with Nrf2.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KEAP1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu327-Cys624 of human KEAP1 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
