(1999) identified a cDNA encoding SLC12A7, which they initially termed KCC3 but later renamed KCC4.
The deduced 1083aa SLC12A7 protein contains 12 membrane-spanning segments, 8 phosphorylation sites, 7 of which are in the C-terminal, and 4 potential N-glycosylation sites
SLC12A7 shares 65% aa identity with SLC12A4 and 66% identity with SLC12A6.
Northern blot analysis detected a 5.3kb SLC12A7 transcript in most tissues tested, with highest expression in heart and kidney and little or no expression in adult brain.
Functional analysis confirmed that SLC12A7 is a KCC.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KCC4.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to His845-Glu1056 of human KCC4 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
