KAZALD1 encodes a secreted member of the insulin growth factor-binding protein (IGFBP) superfamily.
It contains an N-terminal insulin growth factor-binding domain, a central Kazal-type serine protease inhibitor and follistatin-like domain, and a C-terminal immunoglobulin-like domain.
Studies of the mouse ortholog suggest that this gene product may have a function in bone development and bone regeneration.
Bmp2 increased expression of Kazald1 in mouse myoblast, osteoblastic, and mesenchymal stem cell lines, and in primary murine osteoblastic cells.
Addition of recombinant Kazald1 promoted proliferation but not differentiation of osteoblastic cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KAZALD1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg31-Tyr304 of human KAZALD1 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
