Enghild et al.(1989) found that one ITI member, the pre-alpha-trypsin inhibitor (P-alpha-I), is an approximately 130kD protein that contains the mature ITIH3 heavy chain (HC3) and a 30kD subunit.
Bourguignon et al.
(1993) stated that P-alpha-I is composed of HC3 covalently linked to bikunin, a Kunitz-type protease inhibitor.
They reported that the 885aa H3 precursor protein contains a 10aa propeptide and a 19aa signal peptide.
Before or during linkage to bikunin, the C-terminal 238aa are cleaved to produce mature HC3.
H3 shares 54% and 41% identity with the H1 and H2 precursor proteins, respectively.
Diarra-Mehrpour et al.
(1998) corrected the cDNA sequence reported by Bourguignon et al.
(1993).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
