(1987) characterized cDNAs for the H chain of IATI.
Diarra-Mehrpour et al.
(1989) showed that human poly(A)-rich RNAs hybrid-selected with various heavy-chain-encoding cDNA clones translate 3 different heavy chains, designated H1 (M(r) 92,000), H2 (ITIH2), and H3 (ITIH3).
Two previously characterized heavy-chain cDNA clones were found to correspond to the H1 and H2 chains.
The deduced aa sequence of the H3 chain was found to be highly similar to those of the H1(54%) and H2(44%) chains.
Diarra-Mehrpour et al.
(1992) used a PCR-based cloning approach and cDNA library screening to isolate the full-length cDNA H1.
The results of Northern blot analysis by Bost et al.
(1993) indicated that the 2.9-kb ITIH1 mRNA is expressed only in liver.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ITIH1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ser507-Asp819 of human ITIH1 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
