Integrin, beta 5 has been shown to interact with PTK2,Annexin A5and PAK4.
McLean et al.
isolated cDNA clones coding for the beta-5 subunit of the novel integrin receptor involved in cell adhesion to the matrix protein vitronectin which had been found in a human lung epithelial-derived cell line.
This receptor had an alpha subunit that appeared to be identical to that in another vitronectin receptor, but the beta subunit was distinctive.
McLean et al.
demonstrated that the 3.3-kb mRNA coded for a mature protein of 775aa residues with a hydrophobic leader sequence of 24aa.
A 56% identity was found between the beta-5 and beta-3 protein sequences, making them the most closely related of the integrin beta subunits.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse ITGb5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Tyr136-Ile378 of mouse ITGb5 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
