Mechanosensory hair cells within the neuroepithelia of the cochlea and vestibule have stereocilia that are indispensable for mechanosensation.
Evans and Muller (2000) demonstrated that integrin alpha-8/beta-1, its ligand fibronectin, and the integrin-regulated focal adhesion kinase colocalize to the apical hair cell surface where stereocilia are forming
In mice homozygous for a targeted mutation of integrin alpha-8, the colocalization is perturbed, and hair cells in the utricle, a vestibular subcompartment, lack stereocilia or contain malformed stereocilia.
Most integrin alpha-8/beta-1-deficient mice die soon after birth due to kidney defects.
Many of the surviving mice have difficulty balancing, consistent with the structural defects of the inner ear.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ITGa8.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp907-Ala1063 of human ITGa8 fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
