Integrin alpha-5 is product of this gene belongs to the integrin alpha chain family.
Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain.
This gene encodes the integrin alpha 5 chain.
Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor.
In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
Argraves et al.
(1986) isolated cDNA clones coding for the alpha subunit from a placenta cDNA library.
The cDNAs code for 229aa from the C-terminal of the alpha subunit.
The deduced sequence has a hydrophobic region with properties characteristic of a membrane-spanning domain.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse ITGa5.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Glu899-Ala1053 of mouse ITGa5 fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
