(1997) isolated a cDNA for a novel human protein containing both a leucine-rich repeat (LRR) and an immunoglobulin (Ig)-like domain.
ISLR was referred to as ISLR for 'immunoglobulin superfamily containing LRR.' The predicted protein comprises 428aa and contains a hydrophobic N-terminal 18aa putative signal peptide.
Despite the variety of their functions, all members of the Ig superfamily are involved in adhesion or binding to other proteins in solution or at the cell surface.
Therefore it is possible that the ISLR protein may also interact with other proteins or cells.
On Northern blot analysis, the authors found a 2.4-kb ISLR transcript in various human tissues, including retina, heart, skeletal muscle, prostate, ovary, small intestine, thyroid, adrenal cortex, testis, stomach, and spinal cord, as well as fetal lung and fetal kidney.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ISLR.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Cys19-Phe428 of human ISLR fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
