Interferon regulatory factor 3 is a member of the interferon regulatory transcription factor (IRF) family.
IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP.
This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes
The IRF3 gene is present in a single copy in the human genome.
Northern blot analysis detected a 1.6kb constitutively expressed IRF3 transcript.
The deduced 427aa IRF3 protein is 34 to 40% identical to other members of the IRF family in the N-terminal region.
Au et al.
(1995) showed that IRF3 is a 50kD protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse IRF3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Asp406 of mouse IRF3 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
