Ras GTPase-activating-like protein IQGAP1 is a member of the IQGAP family.
The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain.
It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility.
Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.
The IQGAP1 cDNA encodes a 1657aa polypeptide with significant similarity to the Ras-related GTPase-activating (RasGAP) family of proteins.
The N-terminal domain contains 6 copies of a unique motif and 4 IQ motifs (named for the presence of tandem isoleucine and glutamine residues), which are known to modulate binding with calmodulin.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse IQGAP1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gln1276-Lys1657 of mouse IQGAP1 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
