84-4423-49　IPO8 (Importin 8, RANBP8, RAN Binding Protein 8) 200ul　363747

特徴

• Importin 8 is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta.
• This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase
• Human RANBP8 and Xenopus RanBP7 share 61% aa.
• They both belong to a Ran-binding protein superfamily whose members share with importin-beta an N-terminal sequence motif that appears to account for RanGTP binding.
• IPO8 was required for AGO2 binding to a large set of target mRNAs, and knockdown of IPO8 led to a moderate increase of target mRNAs.
• Knockdown of IPO8 had no effect on AGO stability, miRNA biogenesis, or miRNA loading onto AGO proteins.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
• AA Sequence:MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-YQIL TDPNFDPRKK DGALHVIGSL AEILLKKSLF KDQIELFLQN HVFPLLMSNL GYLRARSCWV LHAFSSLKFH NELNLRNAVE LAKKSLIEDE EMPVKVEAAL ALQSLISNQA QAKEHMKPYV RFIMQELLHI VRETENDDVT NVIQKLICEY SQDVASIAVD TTQHLAEIFG KVLQSDEYEE IEDKTVMAMG ILHTIDTILT VV
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.