85kD calcium-independent phospholipase A2 is is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids.
The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells.
Several transcript variants encoding multiple isoforms have been described, but the full-length nature of only two of them have been determined to date.
Tang et al.
(1997) purified an 85kD cytosolic calcium-independent PLA2, termed iPLA2, from Chinese hamster ovary (CHO) cells.
They isolated a partial human cDNA from a Burkitt lymphoma cDNA library by screening with a CHO cDNA.
Northern blot analysis revealed that iPLA2 is expressed in a variety of rodent tissues.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human iPLA2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Arg484-Ile701 of human iPLA2 fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
