84-4423-39 INPP4A (Inositol Polyphosphate-4-Phosphatase Type I 107kD, INPP4, Inositol polyphosphate 4-phosphatase type I, Type I inositol 3, 4-bisphosphate 4-phosphatase) 200ul 363737
84-4423-39 INPP4A (Inositol Polyphosphate-4-Phosphatase Type I 107kD, INPP4, Inositol polyphosphate 4-phosphatase type I, Type I inositol 3, 4-bisphosphate 4-phosphatase) 200ul 363737
NPP4A encodes the inositol polyphosphate 4-phosphatase type I, one of the enzymes involved in phosphatidylinositol signaling pathways.
This enzyme removes the phosphate group at position 4 of the inositol ring from inositol 3,4-bisphosphate
A PCR product was obtained and used to isolate a 5,607-bp composite cDNA which encodes a 939aa reading frame from the rat.
The authors screened a human brain cDNA library and identified a sequence that predicts a 938aa protein which is 97% identical to the rat protein.
Recombinant protein was shown to have the appropriate enzymatic activity.
In the rat, the gene is widely expressed with highest levels in the brain, heart, and skeletal muscle.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes mouse INPP4A.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Mo
Immunogen:Recombinant protein corresponding to Met1-Thr137 of mouse INPP4A fused to His-Tag and T7-tag at N-terminal expressed in E. coli.