IGSF1 contained 12Ig-like domains of the C2 (constant region type 2) type.
In addition, IGSF1 has a signal sequence and a potential transmembrane domain.
IGSF1 was expressed as a 4.7-kb mRNA in many of the tissues tested, with the highest expression in pancreas, testis, and fetal liver.
Additional 2.8- and 5.5-kb transcripts were observed in heart and testis, respectively.
The cDNAs encoded a predicted 1327aa protein that was designated 'immunoglobulin superfamily member-1' (IGSF1) because it contained 12 Ig-like domains of the C2 (constant region type 2) type.
In addition, IGSF1 has a signal sequence and a potential transmembrane domain.
Northern blot analysis revealed that IGSF1 was expressed as a 4.7-kb mRNA in many of the tissues tested, with the highest expression in pancreas, testis, and fetal liver.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-5000Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.