Interleukin 7 is a type I short-chain cytokine belonging to the hematopoietin family.
Mouse IL-7 is an approximately 25kD monomeric glycoprotein that is synthesized as a 154aa residue precursor with a 25aa signal sequence and a 129aa mature segment.
Mature mouse IL-7 has six cysteine residues that form three intrachain disulfide bonds, only one of which is required for bioactivity.
Although multiple alternatively spliced isoforms of human IL-7 that differ in their receptor-binding affinities exist, alternatively spliced isoforms of mouse IL-7 have not been reported.
Cells known to express IL-7 include Class II MHC positive thymic cortical epithelial cells, follicular dendritic cells, intestinal epithelium, monocytes and keratinocytes, endothelial cells, vascular smooth muscle cells and fibroblasts, and bone marrow stromal cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human IL7.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asp26-His177 of human IL7, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
