Interleukin 35 (IL-35) is an IL-12 family cytokine that is produced by regulatory, but not effector, T cells and plays a role in immune suppression.
It is a dimeric protein composed of IL-12alpha and IL-27beta chains, which are encoded by two separate genes called IL12A and EBI3, respectively.
Secreted by regulatory T cells (Tregs), IL-35 suppresses inflammatory responses of immune cells.
Studies in mice show the absence of either IL-35 chain from regulatory Tregs reduces the cells ability to suppress inflammation; this has been observed during cell culture experiments and using an experimental model for inflammatory bowel disease.
To produce its suppressive effects, IL-35 has selective activities on different T cell subsets; it induces proliferation of Treg cell populations but reduces activity of Th17 cell populations.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse IL35.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Arg23-Ala215 of mouse IL35, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
