84-4422-71　IL1RA (Interleukin 1 Receptor Type I, IL1RN, IL1-RA, ICIL1RA, IL1ra3, IL1F3, IRAP, IL-1RA, Interleukin-1 Family Member 3, IL1 inhibitor, Anakinra) 200ul　363670

特徴

• IL-1 receptor antagonist(IL-1RA) is a member of the Interleukin 1 cytokine family and a different type of naturally occurring inhibitor of IL-1 activity, it inhibits IL-1alpha and IL-1beta binding to interleukin receptors.
• A cDNA encoding this polypeptide has been isolated from monocytes and found to code for a mature 152aa residue glycoprotein of 25,000 native molecular weight.
• This molecule, known as secreted IL-1 receptor antagonist, shows 26%aa homology to IL-1beta and 19% homology to IL-1alpha.
• Evidence indicates that the inhibitory action of sIL-1ra results from binding of IL-1ra to the IL-1 receptor Type I with an affinity comparable to that of IL-1alpha or IL-1beta (Kd ~ 200 pM), thus competing with IL-1alpha or IL-1beta for binding to this receptor.
• This binding, however, does not result in signal transduction.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-HPLGK RPCKMQAFRI WDVNQKTFYM RNNQLVAGYL QESNTKLQEK IDVVPIEPDA LFLGLHGRKL CLACVKSGDE IRFQLEAVNI TDLSKNKEEN KRFTFIRSNS GPTTSFESAA CPGWFLCTAQ EADRPVSLTN KPKESFMVTK FYLQEDQ
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.