IL-1 receptor antagonist(IL-1RA) is a member of the Interleukin 1 cytokine family and a different type of naturally occurring inhibitor of IL-1 activity, it inhibits IL-1alpha and IL-1beta binding to interleukin receptors.
A cDNA encoding this polypeptide has been isolated from monocytes and found to code for a mature 152aa residue glycoprotein of 25,000 native molecular weight.
This molecule, known as secreted IL-1 receptor antagonist, shows 26%aa homology to IL-1beta and 19% homology to IL-1alpha.
Evidence indicates that the inhibitory action of sIL-1ra results from binding of IL-1ra to the IL-1 receptor Type I with an affinity comparable to that of IL-1alpha or IL-1beta (Kd ~ 200 pM), thus competing with IL-1alpha or IL-1beta for binding to this receptor.
This binding, however, does not result in signal transduction.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes bovine IL1RA.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Bo
Immunogen：Recombinant protein corresponding to His24-Asp174 of bovine IL1RA, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
