IL1F9 encoded by this gene is a member of the interleukin 1 cytokine family.
The activity of this cytokine is mediated by interleukin 1 receptor-like 2, and is specifically inhibited by interleukin 1 family, member 5 .
Interferon-gamma, tumor necrosis factor-alpha and interleukin 1, beta (IL1B) are reported to stimulate the expression of this cytokine in keratinocytes.
The expression of this cytokine in keratinocytes can also be induced by a contact hypersensitivity reaction or herpes simplex virus infection.
This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.Highly expressed in tissues containing epithelial cells: skin, lung, stomach and esophagus.
In skin is expressed only in keratinocytes but not in fibroblasts, endothelial cells or melanocytes.
Up-regulated in lesional psoriasis skin.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat IL1F9.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Tyr5-Gly153 of rat IL1F9, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
