The cytokine interleukin-1 (IL1) elicits a wide array of biologic activities that initiate and promote the host response to injury or infection by activating a set of transcription factors, including NFKB and AP1, which in turn induce production of effectors of the inflammatory response.
By searching an EST database for sequences resembling IL1 and probing a human genomic library, Smith et al.
(2000) identified a cDNA encoding IL1F6, which they designated FIL1-epsilon.
The deduced 158aa protein, like FIL1-delta, contains neither a signal peptide nor a prodomain.
It shares 48% sequence identity with FIL1-eta.
PCR analyses detected expression in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines.
Modeling indicated that FIL1-epsilon has a conserved 12-stranded beta-trefoil structure.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human IL1e.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Phe158 of human IL1e, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
