Interleukin-1 (IL-1) is one of the first cytokines ever described.
Its initial discovery was as a factor that could induce fever, control lymphocytes, increase the number of bone marrow cells and cause degeneration of bone joints.
At this time, IL-1 was known under several other names including endogenous pyrogen, lymphocyte activating factor, haemopoetin-1 and mononuclear cell factor, amongst others.
It was around 1984-1985 when scientists confirmed that IL-1 was actually composed of two distinct proteins, now called IL-1alpha and IL-1beta.
These belong to a family of cytokines known as the interleukin-1 superfamily.
Both IL-1alpha and IL-1beta are produced by macrophages, monocytes and dendritic cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/mlImmunohistochemistry: 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Positive Control Included:363662A: IL1b, Positive ControlSuppled as a recombinant protein in loading buffer (100mM Tris, pH 6.8, 1% SDS, 150mM sodium chloride, 50mM DTT, 0.02% BPB, 0.02% sodium azide, 50% glycerol)
DAB: 10ul/wellECL: 5ul/wellStore at -20°C.
Stable for 6 months after receipt.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：sheep
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol
Specificity：Recognizes sheep IL1b.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Sh
Immunogen：Recombinant protein corresponding to Met1-Pro266 of sheep IL1b, expressed in E. coli.
