IL-1alpha and IL-1beta recognize the same IL-1 receptor and share a number of similar biological functions.
IL-1alpha is predominantly a cell-associated molecule whereas IL-1beta is a secreted molecule.
IL-1alpha is synthesized primarily as a 31kD precursor that lacks a signal peptide.
Cleavage of the precursor is via the cysteine protease calpain, resulting in a 17.5kD mature IL-1 molecule.
Being active in the processed form, the IL-1 precursor is also biologically active via specific cell binding.
A portion of the precursor is transported to the cell surface and associated with the cell membrane.
Precursor IL-1alpha can be released and cleaved by extracellular proteases when the cells die, and can also be cleaved by activation of the calcium-dependent, membrane-associated calpains.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse IL1a.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Gln101-Ser270 of mouse IL1a, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
