Interleukin 17D is a cytokine that shares the sequence similarity with IL17.
The treatment of endothelial cells with this cytokine has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF.
The increased expression of IL8 induced by this cytokine was found to be NF-kappa B-dependent.The predicted 202aa protein, the longest in the IL17 family, contains a signal peptide, conserved cysteines that allow homodimer formation, and a unique extended C-terminal domain.
RT-PCR analysis showed high expression in skeletal muscle, brain, adipose, heart, lung, and pancreas, with lower expression in bone marrow, fetal liver, kidney, leukocytes, liver, lymph node, placenta, spleen, thymus, tonsil, resting CD4 -positive T cells, and resting CD19 -positive B cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Immunocytochemistry: 1:50-500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat IL17D.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Tyr76-Val274 of rat IL17D, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
