Interleukin-17 (IL17) is a T cell-derived cytokine that may play an important role in the initiation or maintenance of the proinflammatory response.
Shi et al.
(2000) cloned a cDNA encoding IL17B, which they initially designated IL20.
The deduced 184aa protein, 88% identical to the mouse sequence, has a 20aa leader sequence, 1 potential N-glycosylation site, and 8 cysteine residues.
Northern blot analysis detected a 1.0-kb transcript in spinal cord, testis, and small intestine, with less pronounced expression in prostate, colon mucosal lining, and ovary.
SDS-PAGE analysis determined that IL17B is expressed as 23-, 22-, and 18-kD proteins under reducing conditions.
Size-exclusion analysis suggested that IL17B is a nondisulfide-linked dimer.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human IL17B.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gln21-Phe180 of human IL17B, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
