Interleukin 17 (IL-17; also known as IL-17A) is a 30-35kD variably glycosylated homodimeric protein that belongs to a unique family of cysteine-knot related proteins.
Its sequence was originally isolated from an activated rodent hybridoma and termed CTLA-8.
It is synthesized as a 155aa precursor that contains a 23aa signal sequence and a 15kD, 132aa mature segment.
Although there are two intrachain disulfide bonds that create a ring reminiscent of those found in cysteine-knot proteins, the actual closed knot structure does not appear to form.
IL-17 has one potential N-linked glycosylation site.
IL-17, a T cell-derived proinflammatory cytokine, is produced by TCR alpha/Beta+CD4-CD8- thymocytes, as well as by activated CD4+ and CD4+CD45RO+ memory T cells.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat IL17.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ala18-Ser150 of ratIL17, fused to two N-terminal Tags, His-Tag and T7-tag, expressed in E. coli.
