Interleukin 13 (IL-13) is produced primarily by activated T lymphocytes.
It shows approximately 30%aa sequence identity with IL-4 and shares many biological properties with IL-4.
IL-13 has multiple effects on the differentiation and functions of monocytes/macrophages.
It can suppress the cytotoxic functions of monocytes/macrophages and the production of proinflammatory cytokines by monocytes/macrophages.
The biological effects of IL-13 are mediated by specific high-affinity cell surface receptor complexes.
The functional IL-13 receptor complex has been shown to consist of the low-affinity IL-13 receptor alpha chain and the IL-4 receptor alpha subunit (IL-4 Ralpha).
This IL-13 receptor complex may also serve as an alternate high-affinity IL-4 receptor complex in IL-4 responsive cells that lack the gamma chain.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat IL13.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Pro22-His131 of rat IL13, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
