IKK-beta also known as inhibitor of nuclear factor kappa-B kinase subunit beta is an enzyme that serves as a protein subunit of IkappaB kinase, which is a component of the cytokine-activated intracellular signaling pathway involved in triggering immune responses.
Its activity causes activation of a transcription factor known as Nuclear Transcription factor kappa-B or NF-kappaB.
Activated IKK-beta phosphorylates a protein called the inhibitor of NF-kappaB, IkappaB (IkappaBalpha), which binds NF-kappaB to inhibit its function.
Phosphorylated IkappaB is degraded via the ubiquitination pathway, freeing NF-kappaB, and allowing its entry into the nucleus of the cell where it activates various genes involved in inflammation and other immune responses.IKK-beta plays a significant role in brain cells following a stroke.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse IkBKb.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Met1-Trp231 of mouse IkBKb, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
