84-4422-11　IKBIP (Inhibitor Of Nuclear Factor Kappa B Kinase Interacting Protein, IKIP, Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Beta Interacting Protein) 200ul　363610

特徴

• IKIP orthologs are found in other species, including mouse, rat, pig, cow, and Xenopus laevis.
• Mouse and rat Ikip1 and Ikip2 encode 373- and 345aa proteins, respectively, and the human IKIP1 protein shows 77% and 79% identity with mouse and rat Ikip1, respectively.
• Northern blot analysis detected expression of 3 IKIP1 transcripts of 1.3, 1.6 and 2.9 kb in lung, kidney, spleen, thymus, and skeletal muscle.
• Immunofluorescence analysis showed that both transfected and endogenous IKIP1 localizes to the endoplasmic reticulum, and deletion of the hydrophobic region results in uniform distribution of IKIP1 throughout the cell.
• Tunicamycin and endoglycosidase H treatment demonstrated that IKIP1 and IKIP2 are glycosylated, and targeted mutation of IKIP1 showed that glycosylation occurs at asparagine-154.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
• AA Sequence:MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TES SEFQGLQSKI SLISSKYQEC EALVEQLKAF QIAAHLKLLQ EEIHEMKTWS NRITERQGTL NNTLTRLSED IIKVDQGTAS MAKDMGLKIT SVKTDVRRIS GLVTEVESLT DAVQALGNKV KKVETATVEN IGDLLSSSID RTSALRKTAS ENAKRIDSVA QRLAELQGDF DEHTDRFLSL ESDRAKVLKA VSFANDLKPK VSNLKKDFSR LEPLVDDLTL R
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.