IKIP orthologs are found in other species, including mouse, rat, pig, cow, and Xenopus laevis.
Mouse and rat Ikip1 and Ikip2 encode 373- and 345aa proteins, respectively, and the human IKIP1 protein shows 77% and 79% identity with mouse and rat Ikip1, respectively.
Northern blot analysis detected expression of 3 IKIP1 transcripts of 1.3, 1.6 and 2.9 kb in lung, kidney, spleen, thymus, and skeletal muscle.
Immunofluorescence analysis showed that both transfected and endogenous IKIP1 localizes to the endoplasmic reticulum, and deletion of the hydrophobic region results in uniform distribution of IKIP1 throughout the cell.
Tunicamycin and endoglycosidase H treatment demonstrated that IKIP1 and IKIP2 are glycosylated, and targeted mutation of IKIP1 showed that glycosylation occurs at asparagine-154.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat IKBIP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to Ser80-Ile315 of rat IKBIP, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
