IK is in fact part of a larger gene encoding a 557aa protein that is 98% identical to the mouse sequence and contains an extensive stretch of arg-glu-asp (RED) repeats.
Notably, the codon usage in the RED repeat is varied, suggesting the sequence is not a result of repetitive duplication.
RE repeats are found in atrophin-1, and RD repeats are found in RD.
RED is expressed in both bacterial and mammalian cells as an 80kD protein.
Sequence analysis predicted and immunofluorescence analysis demonstrated that RED is localized to nuclear dots similar to the expression pattern of PML bodies; however, RED does not colocalize with PML or coilin (COIL) and appears to be sequestered from the nucleoplasm.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human IK.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met1-Pro192 of human IK, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
