Inositol hexakisphosphate kinase 1 is a protein that belongs to the inositol phosphokinase (IPK) family.
This protein is likely responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5).
It may also convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4.
Alternative splicing occurs for this gene; however, the full-length nature of all transcript variants has not yet been described
By comparison with rat Ihpk1 and database searching, Saiardi et al.
(1999) identified mouse Ihpk1, which is 97% homologous to KIAA0263.
Western blot analysis showed expression of a 50-kD protein.
Northern blot analysis revealed expression of a 5.0-kb transcript in mouse brain and testis, with much lower levels in heart, liver, kidney, lung, and spleen.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity:Recognizes human IHPK1.
Isotype:IgG
Calc Applications Abbrev:E IC IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to Met1-Gly300 of human IHPK1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.