Sequence analysis predicted that the 1,021aa IGSF2 is a type I integral transmembrane protein that has a 20aa hydrophobic signal sequence, a 934aa extracellular domain, a 25aa transmembrane domain, and a short, highly charged 42aa cytoplasmic domain.
The extracellular portion contains 7 pairs of regularly spaced cysteine residues suggestive of a variable type Ig-like protein.
Northern blot analysis revealed strong expression of a 3.6-kb transcript in monocytes, peripheral blood lymphocytes, and lung, and weaker expression in spleen, thymus, and small intestine.
An additional minor transcript of 6.4 kb, which may represent an incompletely spliced transcript, was also seen.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse IGSF2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Leu302-Thr531 of mouse IGSF2, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
