The CMRF35 antigen, which was identified by reactivity with a monoclonal antibody, is present on monocytes, neutrophils, and some T and B lymphocytes (Jackson et al., 1992).
The deduced 224aa type I integral membrane protein contains a 20-residue N-terminal leader sequence, a single V-like Ig extracellular domain, 2 potential N-glycosylation sites, a potentially O-glycosylated hinge-like region, a hydrophobic transmembrane anchor that has a glutamate residue, and a short cytoplasmic tail of 19aa.
Northern blot analysis revealed expression of a 1.4-kb transcript in myeloid and B-lymphoid cell lines and in peripheral blood T cells.
Expression was weak in other cell lines and undetectable in nonlymphoid tissues.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human IGSF16.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Gly21-Arg183 of human IGSF16, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
