McEvoy and Maeda (1988) analyzed the evolutionary history of the haptoglobin gene family by characterizing the haptoglobin genes in primates.
Whereas the HPR gene in the human is 2.2 kb downstream of the HP gene, chimpanzees, gorillas, orangutans, and Old World monkeys have a third gene, which McEvoy and Maeda (1988) named HPP for haptoglobin primate, located 16 kb downstream of HPR.
New World monkeys have only 1 haptoglobin gene.
McEvoy and Maeda (1988) interpreted these observations as suggesting triplication of the haptoglobin locus after divergence of the New World monkeys, followed by deletion of 1 locus in humans.
They stated that, although in vivo transfection experiments indicated that the HPR promoter is active and cell-specific, no hemoglobin-binding protein of the expected structure had been detected.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human HPR.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Asn70-Val332 of human HPR, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
