TCF2 encodes transcription factor 2, a liver-specific factor of the homeobox-containing basic helix-turn-helix family.
The TCF2 protein is believed to form heterodimers with another liver-specific member of this transcription factor family, TCF1; depending on the TCF2 isoform, the result may be to activate or inhibit transcription of target genes.
Mutation of TCF2 that disrupts normal function has been identified as the cause of MODY5.
A third human transcript variant is believed to exist based on such a variant in the rat: however, to date such an mRNA species has not been isolated.
The expression was most prominent in medullary and cortical collecting duct branches, which are ureteric bud derivatives.
TCF2 gene expression was not detected in mesenchymal tissues, suggesting that it plays a role in epithelial differentiation.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human HNF1b.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro159-Phe300 of human HNF1b, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
