High-molecular-weight kininogen (HMWK), is a protein from the blood coagulation system as well as the kinin-kallikrein system.
It is a protein that adsorbs to the surface of biomaterials that come in contact with blood in vivo.
This protein circulates throughout the blood and quickly adsorbs to the material surfaces.
HMWK is one of the early participants of the intrinsic pathway of coagulation, together with Factor XII (Hageman factor) and prekallikrein.
It is 626aa long, and weighs 88-120kD (dependent on glycosylation).
The kininogen is not enzymatically active, and functions only as a cofactor for the activation of kallikrein and Hageman factor.
It is also necessary for the activation of factor XI by factor XIIa.
The histidine-rich region (aa420-510) participates most strongly in coagulation.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse HMWK.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Arg268-Arg540 of mouse HMWK, fused to His-Tag and GST-tag at N-terminal expressed in E. coli.
