High mobility group (HMG) proteins are nonhistone chromosomal proteins.
Seroussi et al.
(1999) characterized a 190.3-kb contig in human 22q13.1 and identified the HMG2L1 and TOM1 genes, as well as the previously identified HMOX1 and MCM5 genes.
(A gene designated THC211630 was later determined to be the same as HMG2L1.
The order of these genes is cen--HMG2L1--TOM1--HMOX1--MCM5--tel.
All are oriented in a 5-prime to 3-prime direction from centromere to telomere.
The HMG2L1 gene contains 6 exons.
The predicted HMG2L1 protein has an HMG box that shares 39%aa sequence identity with the HMG box of bovine HMG2.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human HMG2L1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro264-His540 of human HMG2L1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
