High Mobility Group Box 1is a non-histone chomosomal protein that functions in endotoxin lethality.
It is released from activated macrophages and is present at elevated levels in the serum of sepsis patients.
HMGB1 was also reported to interact with RAGE (receptor for advanced glycation end products) and to influence neuron development.
A large number of sequences related to the cDNA for HMG1 protein hampered the cloning and mapping of the active HMG1 gene.
Ferrari et al.
(1996) showed that the HMG1 gene contains introns, whereas the HMG1-related sequences do not and most likely are retrotransposed pseudogenes.
They identified 8 YACs from the ICI and CEPH libraries that contain the human HMG1 gene.
The gene is similar in structure to the previously characterized mouse homolog.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human HMG1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro9-Arg163 of human HMG1, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
