Hexokinases phosphorylate glucose to produce glucose 6-phosphate, thus committing glucose to the glycolytic pathway.
hexokinase 3.
Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose 6-phosphate.
Furuta et al.
(1996) isolated cDNA clones encoding the HK3 gene from a liver cDNA library.
The sequence encodes a predicted 923aa polypeptide with over 50% identity to human hexokinases I and II.
There was 84.7% identity between the amino acid sequences of human and rat hexokinase III.
Northern blotting showed the presence of hexokinase III mRNA in liver and lung.
By fluorescence in situ hybridization, Furuta et al.
(1996) localized the HK3 gene to 5q35.2.
Colosimo et al.
(1996) used both somatic cell hybrid analysis and fluorescence in situ hybridization to map HK3 to 5q35.3.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human HK3.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Tyr673-Ser903 of human HK3, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
