A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate.
In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product.
Hexokinase-1a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria.
Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency.
Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific.
Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms.
A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-5ug/mlImmunohistochemistry (Paraffin): 5-20ug/mlImmunocytochemistry: 5-20ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A and peptide affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Specificity：Recognizes rat HK1.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to aa413-540 of rat HK1, expressed in E. coli.
