HIST2H2BE encodes a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif
This intronless gene encodes a polypeptide of 126aa.
This H2B gene is paired with a functional H2A histone gene, and the 2 genes are separated by a bidirectionally transcribed intergenic promoter region of 329 nucleotides containing consensus TATA and CCAAT boxes and an OTF-1 element.
The 3-prime untranslated region of the H2B.1 gene contains the conserved histone 3-prime stem-loop motif commonly found in cell cycle-dependent histone genes, as well as a polyadenylation signal typical of constitutively expressed histone genes.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-1:5000Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human HIST2H2BE.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro2-Lys126 of human HIST2H2BE, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
