Acetylation of histone H4 on lysine-16 (H4-K16Ac) is a prevalent and reversible posttranslational chromatin modification in eukaryotes.
Shogren-Knaak et al.
(2006) used a native chemical ligation strategy to generate histone H4 that was homogeneously acetylated at K16.
The incorporation of this modified histone into nucleosomal arrays inhibited the formation of compact 30-nanometer-like fibers and impeded the ability of chromatin to form cross-fiber interactions.
H4-K16Ac also inhibited the ability of the adenosine triphosphate-utilizing chromatin assembly and remodeling enzyme ACF to mobilize a mononucleosome, indicating that this single histone modification modulates both higher order chromatin structure and functional interactions between a nonhistone protein and the chromatin fiber.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes HIST1H4A. Species Crossreactivity: human, mouse, rat, bovine
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Recombinant protein corresponding to Met1-Gly103 of HIST1H4A, fused to His-Tag at N-terminal expressed in E. coli.
