HIP2 belongs to the ubiquitin-conjugating enzyme family.
It binds selectively to a large region at the N terminus of huntingtin.
This interaction is not influenced by the length of the huntingtin polyglutamine tract.
This protein has been implicated in the degradation of huntingtin and suppression of apoptosis.
The 200aa HIP2 protein sequence was identical to that of bovine ubiquitin-conjugating enzyme E2-25K.
Assays of the interaction of HIP2 with huntingtin revealed that HIP2 binds selectively to a large region at the N terminus of huntingtin.
This interaction is not influenced by the length of the huntingtin polyglutamine tract.
Western blot analysis detected protein bands of 25, 28, and 45 kD; the 28-kD band appeared to be selective for tissues of the central nervous system.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human HIP2.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala2-Asn200 of human HIP2, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
