The deduced 349aa HIF1AN protein is homologous to rat Pass1 and to fly and worm sequences, indicating evolutionary conservation of this protein family.
Binding analyses of truncated HIF1AN peptides showed that the inhibitory domain rather than the transactivating domains of HIF1A interacts with HIF1AN, although the presence of TAD-C optimizes binding
Functional analysis indicated that HIAF1AN inhibits HIF1A transcriptional activity in hypoxic and nonhypoxic cells.
Further binding analysis determined that the N terminus of HIF1AN interacts with the N terminus of VHL, and that both interact with HIF1A at distinct sites (VHL with TAD-N of HIF1A), allowing the formation of ternary complexes of the 3 proteins.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human HIF1aN.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Ala2-Asn349 of human HIF1aN, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
