High density lipoprotein-binding protein, also known as vigilin, is a 110-kD protein that specifically binds HDL molecules and may function in the removal of excess cellular cholesterol.
The expression of the gene is under tissue-specific and developmental control.
Vigilin is found in the cytoplasm in close association with the endoplasmic reticulum.
These additional exons, 1A and 1B, are alternatively though concurrently spliced to exon 1C which is homologous to the first exon of the chicken gene.
None of the additional exons code for an N-terminal extension of vigilin, due to in-frame stop codons.
Structural features of exon 1A, however, would allow the translation of the 13aa peptide from an upstream open reading frame preceding the vigilin open reading frame.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human HDLBP.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro158-Ala371 of human HDLBP, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
