Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events.
Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA.
The protein encoded by this gene has sequence homology to members of the histone deacetylase family.
HDAC9 is orthologous to the Xenopus and mouse MITR genes.
The MITR protein lacks the histone deacetylase catalytic domain.
It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs.
This encoded protein may play a role in hematopoiesis.
Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:50-400Immunocytochemistry: 1:50-500Immunohistochemistry (frozen): 1:50-500Immunohistochemistry (paraffin): 1:10-100ELISA: 1:100-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human HDAC9.
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Pro23-Ala343 of human HDAC9, fused to His-Tag and T7-tag at N-terminal expressed in E. coli.
